MICA/MICB Monoklonaler Antikörper

MICA/MICB Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

6D4

Kat-Nr. : PE-65161

Synonyme

MICA, MICB

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Galerie der Validierungsdaten

• 

  FC experiment of HeLa using PE-65161

  1X10^6 HeLa cells were surface stained with 5 ul PE Anti-Human MICA/MICB (PE-65161, Clone:6D4) (red) or unstained (blue). Cells were not fixed.

• 

  FC experiment of HeLa using PE-65161

  1X10^6 HeLa cells were surface stained with 5 ul PE Anti-Human MICA/MICB (PE-65161, Clone:6D4) or unstained. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65161 bindet in FC MICA/MICB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transfizierte MICA-exprimierende C1R-Zellen
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 4276
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.