Ly-6G/Ly-6C (Gr-1) Monoklonaler Antikörper

Ly-6G/Ly-6C (Gr-1) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

2

CloneNo.

RB6-8C5

Kat-Nr. : PE-65140

Synonyme

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse bone marrow cells using PE-65140

  1X10^6 mouse bone marrow cells were surface stained with 0.06 ug PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) (PE-65140, Clone: RB6-8C5). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using PE-65140

  1X10^6 mouse bone marrow cells were surface stained with 0.06 ug PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) (PE-65140, Clone: RB6-8C5). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65140 bindet in FC Ly-6G/Ly-6C (Gr-1) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gegen Granulozyten murinen Ursprungs gezüchtet
• Vollständiger Name: lymphocyte antigen 6 complex, locus G
• GenBank-Zugangsnummer: X70920
• Gene symbol: Ly6g
• Gene ID (NCBI): 546644
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publikationen

Species	Application	Title
		Int J Mol Med; FTY720 attenuates APAP‑induced liver injury via the JAK2/STAT3 signaling pathway.; Authors - Xiangmin He
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang