Lamin B1 Monoklonaler Antikörper

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• KD/KO Validated

Lamin B1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen

1

CloneNo.

3C10G12

Kat-Nr. : CL647-66095

Synonyme

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL647-66095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL647-66095 (Lamin B1 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL647-66095

  1X10^6 HeLa cells were stained with 0.2 ug CoraLite® Plus 647 Anti-Human Lamin B1 (CL647-66095, Clone: 3C10G12) (red), or 0.2 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL647-66095 bindet in IF, FC (Intra) Lamin B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lamin B1 fusion protein Ag20522
• Vollständiger Name: lamin B1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC012295
• Gene symbol: LMNB1
• Gene ID (NCBI): 4001
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Publikationen

Species	Application	Title
human	IF	J Cell Biol; Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.; Authors - Banafsheh Golchoubian

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011