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IMP3 Monoklonaler Antikörper

IMP3 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E7D6

Kat-Nr. : CL647-66247

Synonyme

BRMS2, C15orf12, DKFZp586L0118, FLJ10968, IMP3, MRPS4, U3 snoRNP protein IMP3

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated IMP3 Monoclonal anti (CL647-66247)

    IF Staining of HepG2 using CL647-66247

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 IMP3 antibody (CL647-66247, Clone: 1E7D6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated IMP3 Monoclonal anti (CL647-66247)

    FC experiment of HepG2 using CL647-66247

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IMP3 (CL647-66247, Clone:1E7D6) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-66247 bindet in IF, FC (Intra) IMP3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen IMP3 fusion protein Ag23602
Vollständiger Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Berechnetes Molekulargewicht 184 aa, 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC020557
Gene symbol IMP3
Gene ID (NCBI) 55272
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 IMP3 antibody CL647-66247Protokoll herunterladen
FC protocol for CL Plus 647 IMP3 antibody CL647-66247Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen