IFN gamma Monoklonaler Antikörper

IFN gamma Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

XMG1.2

Kat-Nr. : CL647-65153

Synonyme

Ifg, IFN g, IFN gamma, IFN γ, Ifng, IFN-gamma, IFNγ, IFN-γ, interferon gamma



Geprüfte Anwendungen

Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65153 bindet in FC (Intra) IFN gamma und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name interferon gamma
GenBank-ZugangsnummerBC119063
Gene symbol Ifng
Gene ID (NCBI) 15978
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 647 IFN gamma antibody CL647-65153Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen