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GSK3B Monoklonaler Antikörper

GSK3B Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2E9E4

Kat-Nr. : CL594-67329

Synonyme

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GSK3B Monoclonal antibody (CL594-67329)

    IF Staining of HeLa using CL594-67329

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GSK3B antibody (CL594-67329, Clone: 2E9E4 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated GSK3B Monoclonal antibody (CL594-67329)

    FC experiment of HeLa using CL594-67329

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GSK3B (CL594-67329, Clone:2E9E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67329 bindet in IF, FC (Intra) GSK3B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen GSK3B fusion protein Ag17320
Vollständiger Name glycogen synthase kinase 3 beta
Berechnetes Molekulargewicht 433 aa, 48 kDa
Beobachtetes Molekulargewicht 46-48 kDa
GenBank-ZugangsnummerBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 GSK3B antibody CL594-67329Protokoll herunterladen
FC protocol for CL594 GSK3B antibody CL594-67329Protokoll herunterladen
Standard-Protokolle
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