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Foxp3 Monoklonaler Antikörper

Foxp3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Fluorescent Dye

CloneNo.

3G3

Kat-Nr. : FITC-65089

Synonyme

forkhead box P3, Foxp3, JM2, scurfin, sf

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of mouse splenocytes using FITC Anti-Mouse Foxp3 (3G3) (FITC-65089)

    FC experiment of mouse splenocytes using FITC-65089

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC Foxp3 antibody (FITC-65089, Clone: 3G3).

  • Flow cytometry (FC) experiment of mouse splenocytes using FITC Anti-Mouse Foxp3 (3G3) (FITC-65089)

    FC experiment of mouse splenocytes using FITC-65089

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and then treated with fixation and permeabilization reagents. Cells were then stained with 0.13 ug FITC-mouse IgG1 isotype control antibody.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65089 bindet in FC Foxp3 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name forkhead box P3
GenBank-ZugangsnummerBC132333
Gene symbol Foxp3
Gene ID (NCBI) 20371
Konjugation FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
LagerungsbedingungenBei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Protokolle

Produktspezifische Protokolle
FC protocol for FITC Foxp3 antibody FITC-65089Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen