DYKDDDDK Tag Monoklonaler Antikörper

DYKDDDDK Tag Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IP

Konjugation

HRP Fluorescent Dye

Publikationen

11

CloneNo.

8H6A10

Kat-Nr. : HRP-66008

Synonyme

DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using HRP-66008

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66008 (DYKDDDDK Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IP: See 1 publications below

Produktinformation

HRP-66008 bindet in WB, IP DYKDDDDK Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DYKDDDDK Tag
• Konjugation: HRP Fluorescent Dye
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
	WB	Nat Commun; FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.; Authors - Tingxiang Yan
	WB	Liver Int; The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.; Authors - Peihao Liu
		Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen
	WB	J Bacteriol; Shigella flexneri Diguanylate Cyclases Regulate Virulence.; Authors - Ruchi Ojha
	WB	Biochem Biophys Res Commun; Harnessing coronavirus spike proteins' binding affinity to ACE2 receptor through a novel baculovirus surface display system.; Authors - Lin Wang
	WB	Oncol Lett; Puerarin increases the chemosensitivity of hepatocellular carcinoma cells.; Authors - Zhen Wu