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Desmin Polyklonaler Antikörper

Desmin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-16520

Synonyme

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    IF Staining of mouse heart using CL647-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 Desmin antibody (CL647-16520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    IF Staining of mouse heart using CL647-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 Desmin antibody (CL647-16520) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    FC experiment of C2C12 using CL647-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Desmin (CL647-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMausherzgewebe
Erfolgreiche Detektion in FCC2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-16520 bindet in IF, FC (Intra) Desmin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Desmin fusion protein Ag9742
Vollständiger Name desmin
Berechnetes Molekulargewicht 470 aa, 54 kDa
Beobachtetes Molekulargewicht 52-53 kDa
GenBank-ZugangsnummerBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 Desmin antibody CL647-16520Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen