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DYKDDDDK tag Monoklonaler Antikörper

DYKDDDDK tag Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF

Konjugation

FITC Fluorescent Dye

CloneNo.

8H6A10

Kat-Nr. : FITC-66008

Synonyme

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using FITC-conjugated DYKDDDDK tag Monoclonal antibody ( (FITC-66008)

    IF Staining of Transfected HEK-293 using FITC-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed with Transfected HEK-293 cells using FITC-66008 (DDDDK tag antibody) at dilution of 1:50.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFTransfizierte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-66008 bindet in IF DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Konjugation FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protokolle

Produktspezifische Protokolle
IF protocol for FITC DYKDDDDK tag antibody FITC-66008Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen