CD8a Monoklonaler Antikörper

CD8a Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

53-6.7

Kat-Nr. : CL647-65069

Synonyme

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65069

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD8a (CL647-65069, Clone: 53-6.7) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65069

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65069, Clone:53-6.7) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65069 bindet in IF, FC CD8a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Milzzellen oder Thymozytenmembranen
• Vollständiger Name: CD8 antigen, alpha chain
• GenBank-Zugangsnummer: BC030679
• Gene symbol: Cd8a
• Gene ID (NCBI): 12525
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).