CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen

4

CloneNo.

GL1

Kat-Nr. : FITC-65068

Synonyme

B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using FITC-65068

  1X10^6 LPS treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1) (red) or 0.125 ug FITC-rat IgG2a isotype control. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 4 publications below

Produktinformation

FITC-65068 bindet in FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPS-aktivierte CBA/Ca-Mausmilz-B-Zellen
• Vollständiger Name: CD86 antigen
• GenBank-Zugangsnummer: BC013807
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publikationen

Species	Application	Title
mouse	FC	Carbohydr Polym; Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii.; Authors - Yuqing Sun
mouse	FC	Front Bioeng Biotechnol; Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response; Authors - Xinlong Xie
	FC	Cancer Immunol Immunother; A nanotherapeutic system for gastric cancer suppression by synergistic chemotherapy and immunotherapy based on iPSCs and DCs exosomes; Authors - Yezhou Li
	FC	Neurotoxicology; Ursolic acid promotes microglial polarization toward the M2 phenotype via PPARγ regulation of MMP2 transcription; Authors - Yuye Wang