CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3H5

Kat-Nr. : CL647-65217

Synonyme

B7 1, Cd80, Cd80 molecule

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Galerie der Validierungsdaten

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  FC experiment of wistar rat splenocytes using CL647-65217

  1X10^6 unstimulated (dashed) or LPS-stimulated Wistar rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD80 (CL647-65217, Clone:3H5) (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of wistar rat splenocytes using CL647-65217

  1X10^6 unstimulated (lower) or LPS-stimulated Wistar rat splenocytes (upper) were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD80 (CL647-65217, Clone: 3H5) or 0.5 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS-stimulated Wistar rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65217 bindet in FC CD80 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Cd80 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: XM_017597885
• Gene symbol: Cd80
• Gene ID (NCBI): 25408
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)