CD80 (B7-1) Monoklonaler Antikörper
CD80 (B7-1) Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
PE Fluorescent Dye
CloneNo.
2D10.4
Kat-Nr. : PE-65083
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | Daudi-Zellen, IFN-γ and LPS-stimulated THP-1-derived macrophages |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
PE-65083 bindet in FC CD80 (B7-1) und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG1, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | k.A. |
Vollständiger Name | CD80 molecule |
Berechnetes Molekulargewicht | 33 kDa |
GenBank-Zugangsnummer | BC042665 |
Gene symbol | CD80 |
Gene ID (NCBI) | 941 |
Konjugation | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protokolle
Produktspezifische Protokolle | |
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FC protocol for PE CD80 (B7-1) antibody PE-65083 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |