CD72 Monoklonaler Antikörper

CD72 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG2b

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

3F3

Kat-Nr. : PE-65157

Synonyme

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood lymphocytes using PE-65157

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and 20.00 ul/test PE Anti-Human CD72 (PE-65157, Clone:3F3). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65157

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and PE mouse IgG2b isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65157 bindet in FC CD72 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / Mouse IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Normale humane Lymphozyten aus einem Lymphknoten
• Vollständiger Name: CD72 molecule
• GenBank-Zugangsnummer: BC011734
• Gene symbol: CD72
• Gene ID (NCBI): 971
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).