CD62L (L-Selectin) Monoklonaler Antikörper

CD62L (L-Selectin) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Fluorescent Dye

CloneNo.

MEL-14

Kat-Nr. : FITC-65123

Synonyme

AI528707, CD62L, CD62L (L-Selectin), L selectin, LECAM 1, Lnhr, Ly 22, Ly m22, Lyam 1, Lyam1, selectin, lymphocyte, Sell

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.25 ug FITC-Anti-Mouse CD62L (L-Selectin) (FITC-65123, Clone: MEL-14). Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.25 ug FITC-Rat IgG2a isotype control antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.06 ug FITC Anti-Mouse CD62L (L-Selectin) (FITC-65123, Clone:MEL-14) (red) or 0.06 ug FITC-rat IgG2a isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65123 bindet in FC CD62L (L-Selectin) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/eb-Maus-B-Lymphom 38C-13
• Vollständiger Name: selectin, lymphocyte
• GenBank-Zugangsnummer: BC052681
• Gene symbol: Sell
• Gene ID (NCBI): 20343
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).