CD5 Monoklonaler Antikörper

CD5 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

UCHT2

Kat-Nr. : FITC-65152

Synonyme

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using FITC-65152

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD5 (FITC-65152, Clone: UCHT2) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65152

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD5 (FITC-65152, Clone:UCHT2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65152 bindet in FC CD5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD5 molecule
• Berechnetes Molekulargewicht: 495 aa, 55 kDa
• GenBank-Zugangsnummer: BC027901
• Gene symbol: CD5
• Gene ID (NCBI): 921
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).