CD45R (B220) Monoklonaler Antikörper

CD45R (B220) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

human, Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

RA3-6B2

Kat-Nr. : FITC-65139

Synonyme

B220, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65139

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65139

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC-Rat IgG2a isotype control (left) or 0.5 ug FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65139 bindet in FC CD45R (B220) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines Abelson-Leukämie-virusinduzierte Vorläufer-B-Tumorzellen
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: Ptprc
• Gene ID (NCBI): 19264
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.