CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HI30

Kat-Nr. : CL647-65109

Synonyme

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie der Validierungsdaten

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  IF Staining of Jurkat using CL647-65109

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD45 antibody (CL647-65109, Clone: HI30 ) at dilution of 1:200.

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  FC experiment of human PBMCs using CL647-65109

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone: HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Jurkat-Zellen
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65109 bindet in IF, FC CD45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).