CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

IM7

Kat-Nr. : CL647-65117

Synonyme

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65117

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647 CD44 antibody (CL647-65117, Clone: IM7 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65117

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 1 ug CoraLite® Plus 647 Anti-Mouse CD44 (CL647-65117, Clone: IM7) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65117 bindet in IF, FC CD44 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dexamethason-induzierte myeloische Leukämie-M1-Zellen
• Vollständiger Name: CD44 antigen
• GenBank-Zugangsnummer: BC051388
• Gene symbol: Cd44
• Gene ID (NCBI): 12505
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.