CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

1

CloneNo.

RM4-5

Kat-Nr. : PE-65141

Synonyme

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using PE-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug PE-anti-mouse CD4 (PE-65141, clone RM4-5) (red) or 0.20 ug PE-rat IgG2a isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65141 bindet in FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BALB/c Maus-Thymozyten
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
mouse	FC	J Thromb Haemost; Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1; Authors - Li-Ping Jiang