CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RM4-4

Kat-Nr. : CL647-65199

Synonyme

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using CL647-65199

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Plus Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 1 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65199, Clone: RM4-4) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65199 bindet in FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BALB/c Maus-Thymozyten
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).