CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RM4-5

Kat-Nr. : CL647-65141

Synonyme

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65141

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65141, Clone: RM4-5) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite®488-conjugated Anti-Mouse CD3 (CL488-65077, Clone:17A2) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65141, Clone: RM4-5). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL647-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite®488-conjugated Anti-Mouse CD3 (CL488-65077, Clone:17A2) and CoraLite® Plus 647-conjugated Rat IgG2a isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:250-1:1000

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65141 bindet in IF, FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BALB/c Maus-Thymozyten
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).