CD357 (GITR) Monoklonaler Antikörper

CD357 (GITR) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

DTA-1

Kat-Nr. : PE-65102

Synonyme

AITR, CD357, Gitr, Tnfrsf18

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Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using PE-65102

  1X10^6 mouse splenocytes were surface stained with 0.06 ug APC-Anti-Mouse CD4 (APC-65104, clone GK1.5), 0.125 ug FITC-Anti-Mouse CD25 (FITC-65137, clone PC61.5) and 0.06 ug PE-Anti-Mouse CD357 (GITR) (PE-65102, clone DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65102 bindet in FC CD357 (GITR) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-CD25+-CD4+-T-Zellen
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 18
• GenBank-Zugangsnummer: BC146517
• Gene symbol: Tnfrsf18
• Gene ID (NCBI): 21936
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).