CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

UCHT1

Rezensionen (1)

Kat-Nr. : FITC-65151

Synonyme

CD3, CD3E, T3E, TCRE

---

Galerie der Validierungsdaten

• 

  FC experiment of human PBMCs using FITC-65151

  1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD3 (FITC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated

• 

  FC experiment of human PBMCs using FITC-65151

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated

• 

  FC experiment of human peripheral blood lymphocytes using FITC-65151

  1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug FITC-anti-human CD3 (FITC-65151, clone UCHT1) (red) or 1 ug FITC-mouse IgG1 isotype control (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65151 bindet in FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3E
• Gene ID (NCBI): 916
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Macarena Lucia (Verified Customer) (09-28-2022)

Good staining at recommended dilution

• Applications: Flow Cytometry
• Cell Tissue Type: primary T cell