CD3ε Monoklonaler Antikörper

CD3ε Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Fluorescent Dye

Publikationen

4

CloneNo.

145-2C11

Kat-Nr. : FITC-65060

Synonyme

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65060

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug FITC-Anti-Mouse CD3ε (FITC-65060, clone 145-2C11) (red) or 0.20 ug FITC-Hamster IgG isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 4 publications below

Produktinformation

FITC-65060 bindet in FC CD3ε und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: H-2Kb-spezifischer muriner cytotoxischer T-Lymphozyten (CTL) -Klon BM10-37
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: Cd3e
• Gene ID (NCBI): 12501
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
mouse	FC	Drug Deliv; Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.; Authors - Dingyi Wang
mouse	FC	Asian J Pharm Sci; Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors; Authors - Xudong Fan
mouse	FC	J Thromb Haemost; Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1; Authors - Li-Ping Jiang
mouse	FC	ACS Nano; Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity; Authors - Yunkai Shang