CD3ε Monoklonaler Antikörper

CD3ε Monoklonal Antikörper für FC, IF

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

500-A2

Kat-Nr. : CL647-65061

Synonyme

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65061

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65061

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) (red) or isotype control antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:250-1:1000

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65061 bindet in IF, FC CD3ε und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aus C6VL-BS-Thymomzellen isolierte Immunkomplexe
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: Cd3e
• Gene ID (NCBI): 12501
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).