CD28 Monoklonaler Antikörper
CD28 Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
FITC Plus Fluorescent Dye
CloneNo.
CD28.2
Kat-Nr. : FITC-65099
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | humane PBMCs |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
FITC-65099 bindet in FC CD28 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG1, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | k.A. |
Vollständiger Name | CD28 molecule |
Berechnetes Molekulargewicht | 220 aa, 25 kDa |
GenBank-Zugangsnummer | BC093698 |
Gene symbol | CD28 |
Gene ID (NCBI) | 940 |
Konjugation | FITC Plus Fluorescent Dye |
Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).
Protokolle
Produktspezifische Protokolle | |
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FC protocol for FITC Plus CD28 antibody FITC-65099 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |