CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

PC61.5

Kat-Nr. : PE-65137

Synonyme

CD25, Il2r, Il2ra, Ly 43

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Galerie der Validierungsdaten

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  FC experiment of BALB/c mouse splenocytes using PE-65137

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD4 (APC-65104, Clone: GK1.5) and 0.13 ug PE Anti-Mouse CD25 (PE-65137, Clone: PC61.5). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using PE-65137

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD4 (APC-65104, Clone: GK1.5) and 0.13 ug PE-Rat IgG1 isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65137 bindet in FC CD25 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: Il2ra
• Gene ID (NCBI): 16184
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).