CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen

1

CloneNo.

PC61.5

Kat-Nr. : FITC-65137

Synonyme

CD25, Il2r, Il2ra, Il-2ra, Ly 43

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65137

  1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and 0.13 ug FITC Plus Rat IgG1 isotype control (left) or 0.13 ug FITC Plus Anti-Mouse CD25 (FITC-65137, Clone: PC61.5) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

FITC-65137 bindet in FC CD25 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: Il2ra
• Gene ID (NCBI): 16184
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publikationen

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu