CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

BC96

Kat-Nr. : CL647-65096

Synonyme

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

---

Galerie der Validierungsdaten

• 

  FC experiment of human peripheral blood lymphocytes using CL647-65096

  1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with FITC Anti-Human CD4 (FITC-65134, Clone: OKT4) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD25 (CL647-65096, Clone: BC96). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL647-65096

  1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with FITC Anti-Human CD4 (FITC-65134, Clone: OKT4) and CoraLite® Plus 647-conjugated Mouse IgG1 isotype control antibody. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65096 bindet in FC CD25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• GenBank-Zugangsnummer: BC156627
• Gene symbol: IL2RA
• Gene ID (NCBI): 3559
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).