CD206 Monoklonaler Antikörper

CD206 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

2

CloneNo.

15-2

Kat-Nr. : PE-65155

Synonyme

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Galerie der Validierungsdaten

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  FC experiment of human monocyte-derived dendritic cells using PE-65155

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul PE Anti-Human CD206 (PE-65155, Clone:15-2) (red) or PE-Mouse IgG1 isotype control (black). Cells were not fixed.

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  FC experiment of THP-1 using PE-65155

  THP-1 cells were differentiated into macrophages by incubation in the presence of phorbol 12-myristate 13-acetate (PMA). THP-1-derived macrophages were surface stained with PE Anti-Human CD206 (PE-65155, Clone: 15-2) (dashed line), or polarized in M2 macrophages by incubation with IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 48h and then surface stained with PE Anti-Human CD206 (PE-65155, Clone: 15-2) (red) or isotype control (blue, solid line). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane, aus Monozyten gewonnene dendritische Zellen Zellen, IL-4 and IL-13-stimulated THP-1-derived macrophages

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

PE-65155 bindet in FC CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigter humaner Mannose-Rezeptor
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: MRC1
• Gene ID (NCBI): 4360
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
human	FC	Oncogene; Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells; Authors - Ying Zhan
	FC	Biomed Pharmacother; Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma; Authors - Tsung-Hua Hsieh