CD20 Monoklonaler Antikörper

CD20 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2H7

Kat-Nr. : CL647-65085

Synonyme

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL647-65085

  1X10^6 human PBMCs were surface co-stained with PerCP Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD20 (CL647-65085, Clone: 2H7), or APC Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65085

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD20 (CL647-65085, Clone: 2H7), or APC Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65085 bindet in FC CD20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane tonsillare B-Zellen
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• GenBank-Zugangsnummer: BC002807
• Gene symbol: MS4A1
• Gene ID (NCBI): 931
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).