CD19 Monoklonaler Antikörper

CD19 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4G7

Kat-Nr. : CL647-65197

Synonyme

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL647-65197

  1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 and either 5ul CoraLite® Plus 647 Anti-Human CD19 (CL647-65197, Clone: 4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65197

  1X10^6 human PBMCs were surface stained with 5ul CoraLite® Plus 647 Anti-Human CD19 (CL647-65197, Clone:4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65197 bindet in FC CD19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane chronische lymphatische Leukämie (CLL) -Zellen
• Vollständiger Name: CD19 molecule
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• GenBank-Zugangsnummer: BC006338
• Gene symbol: CD19
• Gene ID (NCBI): 930
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).