CD16 / CD32 Monoklonaler Antikörper

CD16 / CD32 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Fluorescent Dye

Publikationen

2

CloneNo.

2.4G2

Kat-Nr. : FITC-65080

Synonyme

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using FITC-65080

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.25 ug FITC-Rat IgG2b isotype control (left) or 0.25 ug FITC Anti-Mouse CD16 / CD32 (FITC-65080, Clone: 2.4G2) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

FITC-65080 bindet in FC CD16 / CD32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: Fcgr2b
• Gene ID (NCBI): 14130
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).

Publikationen

Species	Application	Title
mouse	FC	Pharm Biol; Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.; Authors - Qi Wang
	FC	Neurotoxicology; Ursolic acid promotes microglial polarization toward the M2 phenotype via PPARγ regulation of MMP2 transcription; Authors - Yuye Wang