CD154 Monoklonaler Antikörper

CD154 Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

MR1

Kat-Nr. : PE-65129

Synonyme

CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes CD4+ Tcell using PE-65129

  CD4+ T cells sorted from splenocytes, were stimulated with PMA and ionomycin and surface stained with 0.13 ug PE Anti-Mouse CD154 (PE-65129, Clone:MR1) (red) or PE-Armenian Hamster IgG isotype control (black). Cells were not fixed.

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  FC experiment of mouse lymphoid cells using PE-65129

  1X10^6 PMA (50 ng/ml) and ionomycin (1 ug/ml)-stimulated mouse lymphoid cells (6 hours) were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 0.06 ug PE Anti-Mouse CD154 (PE-65129, Clone: MR1). Cells were not fixed.

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  FC experiment of mouse lymphoid cells using PE-65129

  1X10^6 PMA (50 ng/ml) and ionomycin (1 ug/ml)-stimulated mouse lymphoid cells (6 hours) were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 0.06 ug PE-Hamster IgG Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: mouse splenocytes CD4+ Tcell

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65129 bindet in FC CD154 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cells
• Vollständiger Name: CD40 ligand
• GenBank-Zugangsnummer: BC119225
• Gene symbol: Cd40lg
• Gene ID (NCBI): 21947
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.