CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

1

CloneNo.

M1/70

Kat-Nr. : PE-65055

Synonyme

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using PE-65055

  1X10^6 mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD11b (PE-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of mouse splenocytes using PE-65055

  1X10^6 mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD11b (PE-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65055 bindet in FC CD11b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/10 Mausmilz-T-Zellen und Concanavalin A-aktivierte C57BL/10-Splenozyten
• Vollständiger Name: integrin alpha M
• GenBank-Zugangsnummer: BC156991
• Gene symbol: Itgam
• Gene ID (NCBI): 16409
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publikationen

Species	Application	Title
mouse	FC	Biomed Pharmacother; Resveratrol alleviated neuroinflammation induced by pseudorabies virus infection through regulating microglial M1/M2 polarization; Authors - Xiangxiu Chen