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CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

H4B4

Kat-Nr. : CL750-65053

Synonyme

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 750 Anti-Human CD107b / LAMP2 (H4B4 (CL750-65053)

    FC experiment of HeLa using CL750-65053

    1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human CD107b (CL750-65053, Clone:H4B4). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65053 bindet in FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Humane adhärente periphere Blutzellen
Vollständiger Name lysosomal-associated membrane protein 2
Berechnetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Konjugation CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).