CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

Kat-Nr. : CL647-65053

Synonyme

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

---

Galerie der Validierungsdaten

• 

  IF Staining of HeLa using CL647-65053

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.

• 

  FC experiment of Jurkat using CL647-65053

  1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65053 bindet in IF, FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 2
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002965
• Gene symbol: LAMP2
• Gene ID (NCBI): 3920
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C