CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

H4A3

Kat-Nr. : FITC-65051

Synonyme

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Galerie der Validierungsdaten

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  IF Staining of HeLa using FITC-65051

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65051 (CD107a antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using FITC-65051

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65051

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65051 bindet in IF, FC CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 1
• GenBank-Zugangsnummer: BC006345
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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