CD103 Monoklonaler Antikörper

CD103 Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

2E7

Kat-Nr. : FITC-65047

Synonyme

Itgae, Integrin alpha M290, Integrin alpha E, aM290, alpha M290

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  FC experiment of mouse splenocytes using FITC-65047

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Anti-Mouse CD103 (2E7) (FITC-65047, Clone:2E7) or 0.25 ug Isotype Control. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65047 bindet in FC CD103 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/6J Maus Intraepitheliale Lymphozyten (IEL)
• Vollständiger Name: integrin alpha E, epithelial-associated
• GenBank-Zugangsnummer: BC150690
• Gene symbol: CD103
• Gene ID (NCBI): 16407
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).