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ATG7 Monoklonaler Antikörper

ATG7 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2B5A10

Kat-Nr. : CL594-67341

Synonyme

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ATG7 Monoclonal antibody (CL594-67341)

    IF Staining of HepG2 using CL594-67341

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 ATG7 antibody (CL594-67341, Clone: 2B5A10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ATG7 Monoclonal antibody (CL594-67341)

    IF Staining of HepG2 using CL594-67341

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 ATG7 antibody (CL594-67341, Clone: 2B5A10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated ATG7 Monoclonal antibody (CL594-67341)

    FC experiment of Jurkat using CL594-67341

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ATG7 (CL594-67341, Clone:2B5A10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67341 bindet in IF, FC (Intra) ATG7 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ATG7 fusion protein Ag29172
Vollständiger Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 78 kDa
Beobachtetes Molekulargewicht 68-78 kDa
GenBank-ZugangsnummerBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 ATG7 antibody CL594-67341Protokoll herunterladen
FC protocol for CL594 ATG7 antibody CL594-67341Protokoll herunterladen
Standard-Protokolle
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