CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MPC-11

Cat no : CL647-65128

Synonyms

CoraLite®647-conjugated Anti-Mouse Isotype Control, gamma2b, IgG2b, IgG2b Isotype Control, Igh-3, IGHG2B

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using CL647-65128

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IgG2b Isotype Control (CL647-65128, Clone:MPC-11) (blue) or CoraLite® Plus 647-conjugated Anti-Human CD4 (CL647-65134, clone: OKT4) (black). Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy chain 3 (serum IgG2b)
• Gene symbol: Igh-3
• Gene ID (NCBI): 16016
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.