MultiPro™ Human Fixed Cell Immune Profiling Antibody cocktail
The MultiPro™ Fixed Cell Immune Profiling Antibody Cocktail contains 58 antibodies. 53 antibodies against cell surface and intracellular proteins plus 5 isotype control antibodies. Fully compatible with the 10x Genomics Single Cell Gene Expression Flex with Feature Barcodes and Sample Multiplexing product.
Host / Isotype
Rabbit polyclonal IgG, Mouse IgG2b, Mouse IgG3, Mouse IgG2a, Mouse IgG1
Reactivity
Human
Cat No : G900004
Validation Data Gallery
Tested Applications
| Positive SC detected in | 10x Genomics Gene Expression with Feature Barcodes and Multiplexing product. |
Recommended dilution
Lyophilized cocktail reconstituted in 55uL of IC buffer can be used to stain up to 1 million cells (see protocol)
Product Information
| Tested Reactivity | Human |
| Host / Isotype | Rabbit polyclonal IgG, Mouse IgG2b, Mouse IgG3, Mouse IgG2a, Mouse IgG1 |
| Class | Monoclonal and Polyclonal |
| Type | Antibody |
| Storage Buffer | Lyophilized antibody cocktail |
| Storage Conditions | Store at 2-8°C. |
Background Information
Contains 53 antibodies against cellular targets and 5 isotype control antibodies. The cocktail is compatible with the 10x Genomics Gene Expression with Feature Barcodes and Multiplexing product.
Targets
| Nuclear | Clone | Ensembl ID | Uniprot ID | Expressed by* |
| AHNAK | Polyclonal | ENSG00000124942 | Q09666 | DCs, T cells, basophils |
| BACH1 | 2D12A4 | ENSG00000156273 | O14867 | Neutrophils, APCs, NK cells |
| IRF4 | Polyclonal | ENSG00000137265 | Q15306 | T cells, APCs |
| MAFB | Polyclonal | ENSG00000204103 | Q9Y5Q3 | Monocytes/Macrophages |
| NFKB2 | 6A10E9 | ENSG00000077150 | Q00653 | Ubiquitous |
| POU2AF1 | 3C5A7 | ENSG00000110777 | Q16633 | B cells, Airway epithelial cells |
| SPI1 | 2H3D3 | ENSG00000066336 | P17947 | Granulocytes, Monocytes, B cells, NK cells |
| T-bet | Polyclonal | ENSG00000073861 | Q9UL17 | NK cells, T cells, B cells |
| Cytosolic | Clone | Ensembl ID | Uniprot ID | Expressed by* |
| AIF1 | Polyclonal | ENSG00000204472 | P55008 | APCs, T cells |
| LASP1 | 1G4B6 | ENSG00000002834 | Q14847 | Leukocytes |
| SRC | 5E10C4 | ENSG00000197122 | P12931 | T cells, B cells |
| SYK | 4C4A12 | ENSG00000165025 | P43405 | Monocytes, B cells, NK cells |
| VIM | 3H9D1 | ENSG00000026025 | P08670 | Mesenchymal cells |
| ZAP70 | 1A11G9 | ENSG00000115085 | P43403 | NK cells, T cells, B cells |
| Secreted | Clone | Ensembl ID | Uniprot ID | Expressed by* |
| IFNG | 1E10G7 | ENSG00000111537 | P01579 | T cells, NK cells, APCs, ILCs |
| IL-1B | 2A1B4 | ENSG00000125538 | P01584 | Neutrophils, APCs |
| IL-17A | 1F3E3 | ENSG00000112115 | Q16552 | Th17 cells, ILCs, NK cells |
| IL-6 | 4G3E6 | ENSG00000136244 | P05231 | T cells, APCs, fibroblasts |
| IL-8 | Polyclonal | ENSG00000169429 | P10145 | APCs, Epithelial cells, T cells, APCs |
| S100A4 | 2G11B4 | ENSG00000196154 | P26447 | Leukocytes |
| TGFBI | 3E11D11 | ENSG00000120708 | Q15582 | Leukocytes |
| TNFa | 7B8A11 | ENSG00000232810 | P01375 | APCs, T cells, NK cells |
| Cell Surface | Clone | Ensembl ID | Uniprot ID | Expressed by* |
| ANXA2 | 1C1E12 | ENSG00000182718 | P07355 | Basophils, Macrophages, T cells, DCs |
| CD11a | TS2/4 | ENSG00000005844 | P20701 | Leukocytes |
| CD11b | ICRF44 | ENSG00000169896 | P11215 | Granulocytes, Monocytes/Macrophages |
| CD11c | 3.9 | ENSG00000140678 | P20702 | DCs, B cells |
| CD13 | WM15 | ENSG00000166825 | P15144 | Granulocytes, Monocytes |
| CD16 | 3G8 | ENSG00000203747 | P08637 | NK cells, Monocytes, Neutrophils |
| CD18 | TS1/18 | ENSG00000160255 | P05107 | Leukocytes |
| CD19 | HIB19 | ENSG00000177455 | P15391 | B cells |
| CD2 | TS1/8 | ENSG00000116824 | P06729 | NK cells, T cells |
| CD21 | BU32 | ENSG00000117322 | P20023 | B cells |
| CD27 | 1C1G3 | ENSG00000139193 | P26842 | Treg, T cells, B cells |
| CD28 | CD28.2 | ENSG00000178562 | P10747 | T cells, NK cells |
| CD35 | E11 | ENSG00000203710 | P17927 | B cells, T cells, Monocytes, Granulocytes |
| CD3E | OKT3 | ENSG00000198851 | P07766 | T cells |
| CD4 | RPA-T4 | ENSG00000010610 | P01730 | pDCs, Monocytes, mDCs, T cells |
| CD40 | G28.5 | ENSG00000101017 | P25942 | APCs |
| CD43 | 2A11D6 | ENSG00000197471 | P16150 | Leukocytes |
| CD44 | F10-44-2 | ENSG00000026508 | P16070 | Ubiquitous |
| CD45 | HI30 | ENSG00000081237 | P08575 | Lymphocytes |
| CD45RA | F8-11-13 | ENSG00000081237 | P08575 | B and T cells |
| CD5 | UCHT2 | ENSG00000110448 | P06127 | T cells |
| CD54 | 15.2 | ENSG00000090339 | P05362 | Monocytes, NK cells, DCs |
| CD63 | TS63 | ENSG00000135404 | P08962 | Basophils, Monocytes, NK cells, T cells |
| CD64 | 10.1 | ENSG00000150337 | P12314 | Monocytes, DCs |
| CD7 | 4H9 | ENSG00000173762 | P09564 | NK cell, T cell |
| CD71 | 3C11F11 | ENSG00000072274 | P02786 | Monocytes, B cells |
| CD81 | 5A6 | ENSG00000110651 | P60033 | Leukocytes |
| CD83 | HB15e | ENSG00000112149 | Q0151 | APC B cells |
| CD86 | BU63 | ENSG00000114013 | P42081 | Monocytes, B cells |
| CD8A | UCHT4 | ENSG00000153563 | P01732 | T cells |
| HLA-DRA | L243 | ENSG00000204287 | P01903 | APCs |
| Controls | Clone | |||
|---|---|---|---|---|
| Rabbit IgG | Polyclonal | |||
| Mouse IgG2b | 11B8C4 | |||
| Mouse IgG3 | 1H4A5 | |||
| Mouse IgG2a | 11A1B2 | |||
| Mouse IgG1 | 1F8D3 |
APCs = Antigen Presenting Cells, ILCs = Innate Lyphoid Cells
DCs = Dendritic Cells, NK = Natural Killer Cells
* Non-exhaustive list
Protocols
| Download Protocol |
Sequencing Parameters
IMPORTANT: The sequencing parameters for MultiPro™ are distinct from those required for 10x Genomics Gene Expression Flex sequencing libraries.
MultiPro™ sequencing parameters are also distinct from other commercially available oligonucleotide conjugated antibodies. Please be mindful of this when setting up your sequencing run. The required MultiPro™ sequencing parameters are:
If using Cell Ranger v8.0+
Read 1: 28 cycles
Index 1:10 cycles
Index 2:10 cycles
Read 2:90 cycles
If using Cell Ranger v7.2
Read 1: 48 cycles
Index 1:10 cycles
Index 2:10 cycles
Read 2: 50 cycles