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MultiPro® HashMax™-5 Cell Hashing Antibodies

The MultiPro® HashMax™-5 Cell Hashing DNA-barcoded Antibody Kits offer a cost-effective solution for single-cell sequencing, while significantly enhancing experimental flexibility and performance. This advanced approach improves cell recovery rates, reduces undetected multiplet rates, and enables effective hashing across diverse sample types. The kits are available as two separate sets, each containing 4 unique HashMax™-5 hashcodes, or as a complete set of all 8 hashcodes (10 reactions per hashcode). Fully validated for use with 10x Genomics 5' Gene Expression and Flex Fixed RNA Profiling kits, HashMax™-5 ensures robust, high-quality single-cell analysis with unparalleled precision and reliability.

Host / Isotype

Mouse IgG1

Reactivity

Human

Oligo Format

HashMax-5

Applications

Sample Multiplexing, Cell Hashing

Recommended Dilution

2 ul/sample

Cat No : G900081

Validation Data Gallery

  • Left Panel: Human PBMCs stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics 5' Gene Expression workflow. Image shows successful antibody based demultiplexing. 4 unique donors were used, each donor was split into two samples and stained with cell hashing antibodies. Right Panel: Demultiplexing of human PBMCs based on SNP profiles. Each donor is represented by two clusters.

    Left Panel: Human PBMCs stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics 5' Gene Expression workflow. Image shows successful antibody based demultiplexing. 4 unique donors were used, each donor was split into two samples and stained with cell hashing antibodies.
    Right Panel: Demultiplexing of human PBMCs based on SNP profiles. Each donor is represented by two clusters.

  • Left Panel: Cells from various cells were stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics 5' Gene Expression workflow. Image shows successful antibody based demultiplexing. CD34+ bone marrow cells were included in this analysis. Right Panel: Expression of CD34 gene, demonstrating successful staining of progenitor cells.

    Left Panel: Cells from various cells were stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics 5' Gene Expression workflow. Image shows successful antibody based demultiplexing. CD34+ bone marrow cells were included in this analysis.
    Right Panel: Expression of CD34 gene, demonstrating successful staining of progenitor cells.

  • Differentiated iPSCs stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics Flex workflow.

    Differentiated iPSCs stained with MultiPro® HashMax™-5 antibodies and processed through the 10x Genomics Flex workflow.

Product Information

The MultiPro® HashMax-5 Antibodies include a mix of anti-CD29 (TS2/16) and anti-B2M (1H3F4), designed to enable effective cell hashing across a wide range of primary cell types and cell lines.

Tested Reactivity Human
Host / Isotype Mouse IgG1
Class Monoclonal
Type Antibody
Storage Buffer Glycerol Buffer
Storage ConditionsStore at -20°C.

Targets

CD29 (TS2/16), B2M (1H3F4)

HashKit 1 hashcodes A-D, components

ComponentSize
MultiPro® HashMax™-5, Hashcode AG900123-10RXNS
MultiPro® HashMax™-5, Hashcode BG900124-10RXNS
MultiPro® HashMax™-5, Hashcode CG900125-10RXNS
MultiPro® HashMax™-5, Hashcode DG900126-10RXNS

HashKit 2 hashcodes E-H, components

ComponentSize
MultiPro® HashMax™-5, Hashcode EG900127-10RXNS
MultiPro® HashMax™-5, Hashcode FG900128-10RXNS
MultiPro® HashMax™-5, Hashcode GG900129-10RXNS
MultiPro® HashMax™-5, Hashcode HG900130-10RXNS

HashKit 3 hashcodes A-H, components

ComponentSize
MultiPro® HashMax™-5, Hashcode AG900123-10RXNS
MultiPro® HashMax™-5, Hashcode BG900124-10RXNS
MultiPro® HashMax™-5, Hashcode CG900125-10RXNS
MultiPro® HashMax™-5, Hashcode DG900126-10RXNS
MultiPro® HashMax™-5, Hashcode EG900127-10RXNS
MultiPro® HashMax™-5, Hashcode FG900128-10RXNS
MultiPro® HashMax™-5, Hashcode GG900129-10RXNS
MultiPro® HashMax™-5, Hashcode HG900130-10RXNS

Background Information

MultiPro® HashMax™-5 Antibodies offer versatile applications, including increasing target cell recovery, reducing the undetected multiplet rate, and enabling efficient analysis of limited cell samples when used with 10x Genomics Flex Gene Expression and 5’ Gene Expression profiling. Unlike other sample multiplexing solutions on the market, HashMax provides the unique advantage of independent hashtag library generation, offering enhanced control over sequencing outputs and significant improvements in overall data quality.

Protocols

Cell Hashing Protocol for FlexDownload Protocol
Cell Hashing Protocol for 5' Gene ExpressionDownload Protocol