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EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6E6E6

Cat no : 66071-1-Ig

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig

    WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HeLa using 66071-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of human brain using 66071-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of COLO 320 using 66071-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    IF Staining of HeLa using 66071-1-Ig

    Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPLIN fusion protein Ag13288
Full Name LIM domain and actin binding 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa, 110 kDa
GenBank Accession NumberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Protocols

Product Specific Protocols
WB protocol for EPLIN antibody 66071-1-IgDownload protocol
IHC protocol for EPLIN antibody 66071-1-IgDownload protocol
IF protocol for EPLIN antibody 66071-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols