CoraLite® Plus 488-conjugated EPLIN Monoclonal antibody

• Featured Product
• KD/KO Validated

EPLIN Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6E6E6

Cat no : CL488-66071

Synonyms

EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-66071

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66071 (EPLIN antibody) at dilution of 1:100.

---

Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66071 is EPLIN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPLIN fusion protein Ag13288
• Full Name: LIM domain and actin binding 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC001247
• Gene Symbol: LIMA1
• Gene ID (NCBI): 51474
• RRID: AB_2883250
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.