CoraLite® Plus 750-conjugated c-Cbl Monoclonal antibody

• Featured Product
• KD/KO Validated

c-Cbl Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2F6D1

Cat no : CL750-66576

Synonyms

C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55

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Validation Data Gallery

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  WB analysis using CL750-66576

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66576 (c-Cbl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, mouse brain tissue, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-Cbl fusion protein Ag23033
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC132733
• Gene Symbol: CBL
• Gene ID (NCBI): 867
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).