WBP11 Polyclonal antibody

WBP11 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10316-1-AP

Synonyms

SIPP1, SNP70, WBP 11, WBP11, WW domain binding protein 11

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Validation Data Gallery

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  WB analysis using 10316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10316-1-AP (WBP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10316-1-AP

  IP result of anti-WBP11 (IP:10316-1-AP, 3ug; Detection:10316-1-AP 1:500) with mouse heart tissue lysate 6000ug.

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  IHC staining of human breast cancer using 10316-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10316-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10316-1-AP

  Immunofluorescent analysis of HeLa cells using 10316-1-AP (WBP11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 10316-1-AP is WBP11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBP11 fusion protein Ag0366
• Full Name: WW domain binding protein 11
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC001621
• Gene Symbol: WBP11
• Gene ID (NCBI): 51729
• RRID: AB_10694664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.