CoraLite® Plus 647-conjugated WASF3 Monoclonal antibody

WASF3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3B7D10

Cat no : CL647-67620

Synonyms

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

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Validation Data Gallery

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  IF Staining of mouse brain using CL647-67620

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 WASF3 antibody (CL647-67620, Clone: 3B7D10 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67620 targets WASF3 in IF applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WASF3 fusion protein Ag29991
• Full Name: WAS protein family, member 3
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 and 66-70 kDa
• GenBank Accession Number: BC050283
• Gene Symbol: WASF3
• Gene ID (NCBI): 10810
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASF3, also named as WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with the movement, invasion and metastasis in different cancers such as breast cancer, gastric cancer and prostate cancer. It has been shown that WASF3 levels are elevated in advanced stage cancers compared to lower grade tumors and normal tissue. 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa protein similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)